Cardiac Biomarker Proteins (Reagents)
Recombinant Cardiac Reference Proteins for Calibrators, Controls, and Assay Development
Spectral Medical has manufactured recombinant cardiac biomarker proteins for over 25 years, supplying diagnostic instrument manufacturers, IVD kit developers, and research laboratories globally.
Our cardiac protein portfolio covers the two most clinically significant biomarker categories in cardiovascular testing: cardiac Troponin (cTnI-C and cTnI-C-II) and NT-proBNP.
Recombinant Cardiac Biomarker Proteins
RP 3500
Single-Chain Cardiac Troponin I-C (ScTnI-C-1)
Human cTnI-cTnC fusion protein. Approximately 44 kDa. Purity above 85% by SDS-PAGE.
First-generation single-chain Troponin I-C.
Established reference protein for immunoassay and western blot applications.
RP 3700 Single-Chain Cardiac Troponin I-C Second Generation (ScTnI-C-2)
Human cTnI (aa 28-110) fused to cTnC. Approximately 30 kDa. Purity above 95% by SDS-PAGE.
Second-generation single-chain Troponin I-C.
Highest-specification Troponin I-C product in the
Spectral range.
RP 8100
Recombinant NT-ProBNP
Human NT-proBNP amino acids 1-76, His-tagged. Approximately 9 kDa per subunit. Purity above 90% by SDS-PAGE.
Recombinant amino-terminal fragment of human brain natriuretic peptide precursor. For cardiac panel programmes covering heart failure.
All proteins are expressed in E. coli and contain no animal or human-derived components. Available in 0.1 mg, 1.0 mg, 5.0 mg, and 10.0 mg. Bulk quantities available by special order.
All Spectral Reagent Products are for research use only. Not for human or in vivo use.
Contact us for pricing. Email: reagents@spectraldx.com Phone: 416‑626‑3233 ext. 2200
Dual Analyte Detection: Free cardiac Troponin I (cTnI) is intrinsically unstable and progressively loses immunoreactivity in solution, while most troponin reference materials contain either Troponin I or Troponin C alone. Spectral’s single-chain Troponin I–C proteins (RP-3500 and RP-3700) covalently link cTnI and cTnC into a single molecule, preserving immunoreactivity, enabling dual antibody recognition with fixed stoichiometry, and providing a more stable and consistent reference material across multiple assay formats [Liu et al., Clin Lab. 2001;47(1–2):19–27. PMID: 11214219].
Product Applications
Calibrators for Immunoassays
RP-3500 and RP-3700 are used to build calibration curves in troponin-based immunoassays. RP-8100 serves the same function in NT-proBNP assays.
Controls for Immunoassays
The same proteins are used as quality control materials alongside calibrators to monitor assay performance across testing runs
Western Blot Standards
RP-3700 at approximately 30 kDa and above 95% purity provides a defined reference band for troponin detection. RP-8100 at approximately 9 kDa per subunit suits low-molecular-weight NT-proBNP detection applications.
Why Spectral Medical
25 Years of Manufacturing
Spectral has produced cardiac biomarker proteins for over 25 years. That depth of experience is built into every production run and every supply commitment.
No Animal or Human Components
All proteins are produced by E. coli recombinant expression. No animal-derived or human-derived materials are used in any product.
Consistent Purity
Purity above 85% for RP-3500, above 95% for RP-3700, and above 90% for RP-8100, delivered by affinity purification with Certificate of Analysis (CoA) available per production lot.
Long Shelf Life
Spectral proteins are recognised in the market for their long shelf life, reducing inventory pressure and recalibration frequency for instrument platforms and kit programmes.
Flexible Order Sizes
From 0.1 mg for initial assessment through to bulk commercial supply under a Material Supply Agreement. One qualified supplier at every stage of your programme.
Supply Agreement Ready
Recombinant Proteins Frequently Asked Questions
What are these proteins used for?
What is the difference between RP-3500 and RP-3700?
Do these proteins contain animal or human-derived components?
All Spectral cardiac proteins are produced by E. coli recombinant expression and contain no animal-derived or human-derived components.
What quantities are available?
Can Spectral support a long-term supply agreement?
Material Supply Agreement and Quality Agreement frameworks are already in place. Spectral supplies from initial assessment quantities through to bulk commercial volumes with the same qualified material throughout, supporting programmes from early development through to full-scale production.