Cardiac Biomarker Proteins (Reagents)

Recombinant Cardiac Reference Proteins for Calibrators, Controls, and Assay Development

Spectral Medical manufactures recombinant cardiac biomarker proteins for immunoassay development, IVD calibration, assay controls, and diagnostic research applications.

For more than 25 years, Spectral has supplied recombinant troponin and NT-proBNP proteins to diagnostic instrument manufacturers, IVD kit developers, OEM partners, and research laboratories worldwide.

Our recombinant cardiac biomarkers are designed to support assay reproducibility and analytical performance across a range of cardiovascular diagnostic applications.

Product Brochure

Recombinant Cardiac Biomarker Proteins

RP 3500
Single-Chain Cardiac Troponin I-C (ScTnI-C-1)

Human cTnI-cTnC fusion protein. Approximately 44 kDa. Purity above 85% by SDS-PAGE.

First-generation single-chain Troponin I-C.
Established reference protein for immunoassay and western blot applications.

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RP 3700 Single-Chain Cardiac Troponin I-C Second Generation (ScTnI-C-2)

Human cTnI (aa 28-110) fused to cTnC. Approximately 30 kDa. Purity above 95% by SDS-PAGE.

Second-generation single-chain Troponin I-C.
Highest-specification Troponin I-C product in the
Spectral range.

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RP 8100
Recombinant NT-ProBNP

Human NT-proBNP amino acids 1-76, His-tagged. Approximately 9 kDa per subunit. Purity above 90% by SDS-PAGE.

Recombinant amino-terminal fragment of human brain natriuretic peptide precursor. For cardiac panel programmes covering heart failure.

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All proteins are expressed in E. coli and contain no animal or human-derived components. Available in 0.1 mg, 1.0 mg, 5.0 mg, and 10.0 mg. Bulk quantities available by special order.
All Spectral Reagent Products are for research use only. Not for human or in vivo use.

Contact us for pricing. Email: reagents@spectraldx.com Phone: 416‑626‑3233 ext. 2200

Dual Analyte Detection: Free cardiac Troponin I (cTnI) is intrinsically unstable and progressively loses immunoreactivity in solution, while most troponin reference materials contain either Troponin I or Troponin C alone. Spectral’s single-chain Troponin I–C proteins (RP-3500 and RP-3700) covalently link cTnI and cTnC into a single molecule, preserving immunoreactivity, enabling dual antibody recognition with fixed stoichiometry, and providing a more stable and consistent reference material across multiple assay formats [Liu et al., Clin Lab. 2001;47(1–2):19–27. PMID: 11214219].

Product Applications

For Immunoassays

RP-3500 and RP-3700 are used as calibrators and control materials in troponin-based immunoassays, while RP-8100 performs the same functions in NT-proBNP assays.

These recombinant proteins support the generation of accurate calibration curves and provide reliable quality control, helping ensure consistent assay performance across testing runs.

For IVD Development

These recombinant proteins are designed to support all stages of in vitro diagnostic (IVD) assay development, from early feasibility studies through to validation and commercialization. Their high purity, well-defined structure, and consistent biochemical properties enable reliable calibration, analytical evaluation, and reproducibility, helping developers achieve the sensitivity, specificity, and lot-to-lot consistency required for robust diagnostic assays.

For Western Blotting

The same proteins can be used as quality control materials and reference standards in Western blot applications to monitor assay performance. RP-3700, at approximately 30 kDa and >95% purity, provides a clear reference band for troponin detection, while RP-8100, at approximately 9 kDa per subunit, is well suited for low-molecular-weight NT-proBNP detection.

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Why Diagnostic Developers Choose Spectral

25 Years of Manufacturing

Spectral has produced cardiac biomarker proteins for over 25 years. That depth of experience is built into every production run and every supply commitment.

No Animal or Human Components

All proteins are produced by E. coli recombinant expression. No animal-derived or human-derived materials are used in any product.

Consistent Purity

Purity above 85% for RP-3500, above 95% for RP-3700, and above 90% for RP-8100, delivered by affinity purification with Certificate of Analysis (CoA) available per production lot.

Long Shelf Life

Spectral proteins are recognised in the market for their long shelf life, reducing inventory pressure and recalibration frequency for instrument platforms and kit programmes.

Flexible Order Sizes

From 0.1 mg for initial assessment through to bulk commercial supply under a Material Supply Agreement. One qualified supplier at every stage of your programme.

Supply Agreement Ready

Material Supply Agreement and Quality Agreement frameworks are in place and can be adapted to your organisation’s requirements.

Cardiac Biomarker Proteins – Frequently Asked Questions

What are recombinant cardiac biomarker proteins used for?

Recombinant cardiac biomarker proteins are used as calibrators, controls, and reference materials in diagnostic assays such as troponin and NT-proBNP immunoassays. They enable accurate quantification of cardiac biomarkers and support consistent assay performance.

These proteins are widely used across diagnostic instrument platforms, IVD kit development, and laboratory research applications.

What is the difference between RP-3500 and RP-3700 recombinant troponin proteins?

RP-3500 and RP-3700 are both recombinant Troponin I-C fusion proteins expressed in E. coli, but differ in purity and design. RP-3500 is a first-generation protein (~44 kDa) with >85% purity, while RP-3700 is a second-generation, affinity-purified recombinant protein (~30 kDa) with >95% purity.

RP-3700 incorporates a refined Troponin I fragment (amino acids 28–110) fused to Troponin C, making it the preferred choice for applications requiring higher purity and performance, such as sensitive immunoassays and IVD development.

Do these recombinant proteins contain animal- or human-derived materials?

No, all Spectral recombinant cardiac biomarker proteins are produced using E. coli expression systems and contain no animal-derived or human-derived components. This ensures high consistency, reduced variability, and suitability for regulated diagnostic applications, including IVD manufacturing.

Are bulk quantities available for OEM and IVD manufacturing?

Yes, Spectral offers bulk quantities of recombinant cardiac biomarker proteins to support OEM and IVD manufacturing requirements. Standard sizes range from 0.1 mg to 10 mg, with larger volumes available under customized supply agreements. This flexibility supports both early-stage development and large-scale commercial production.

Can Spectral support long-term OEM or supply agreements?

Yes, Spectral supports long-term supply through established Material Supply Agreements and Quality Agreements. These frameworks enable consistent supply of qualified recombinant proteins from initial evaluation through to full-scale manufacturing.

What quality and technical documentation is available?

Each production lot is supplied with a Certificate of Analysis (CoA) confirming key parameters such as purity and concentration. Additional technical documentation can be provided to support assay development, validation, and regulatory submissions.

Spectral works with customers to meet specific documentation requirements for IVD and OEM applications.

What are the recommended storage and stability conditions?

Spectral recombinant cardiac biomarker proteins should be stored at -20°C to maintain stability and performance.

These proteins are designed for long shelf life under recommended storage conditions, supporting consistent use across extended development and manufacturing timelines.

How can I request product specifications or pricing information?

Product specifications and pricing information can be obtained by contacting Spectral directly. Pricing is typically based on order volume, with discounts available for bulk purchases and long-term supply agreements.

Please reach out to slahiri@spectraldx.com to discuss your specific requirements.

Cardiac Biomarker Insights